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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
25/09/2016 |
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Actualizado : |
09/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ARRUABARRENA, A.; BENITEZ-GALEANO, M.J.; GIAMBIASI, M.; BERTALMIO, A.; COLINA, R.; HERNÁNDEZ-RODRÍGUEZ, L. |
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Afiliación : |
ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA JOSÉ BENÍTEZ-GALEANO, Laboratorio de Virología Molecular, Centro Universitario Regional Noroeste (CENUR Noroeste), Universidad de la República; MARIO ALEJANDRO GIAMBIASI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA MARIA BERTALMIO CASARIEGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODNEY COLINA, Laboratorio de Virología Molecular, Centro Universitario Regional Noroeste (CENUR Noroeste), Universidad de la República; LESTER HERNÁNDEZ-RODRÍGUEZ, Instituto de Investigaciones en Fruticultura Tropical, La Habana, Cuba. |
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Título : |
Application of a simple and affordable protocol for isolating plant total nucleic acids for RNA and DNA virus detection. |
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Fecha de publicación : |
2016 |
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Fuente / Imprenta : |
Journal of Virological Methods, 2016, v.237, p. 14-17. |
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DOI : |
10.1016/j.jviromet.2016.08.011 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 30 May 2016 / Received in revised form 26 July 2016 / Accepted 14 August 2016 / Available online 16 August 2016. |
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Contenido : |
ABSTRACT.
Standard molecular methods for plant virus diagnosis require the purification of RNA or DNA extracts from a large number of samples, with sufficient concentration and quality for their use in PCR, RT-PCR, or qPCR analysis. Most methods are laborious and use either hazardous and/or costly chemicals.Apreviously published protocol for RNA isolation from several plant species yields high amounts of good quality RNADNA mixture in a simple, safe and inexpensive manner. In the present work, this method was tested to obtain RNA-DNA extracts from leaves of tomato, potato and three species of citrus, and was compared with two commercial kits. The results demonstrated that this protocol offers at least comparable nucleic
acid quality, quantity and purity to those provided by commercial phenol-based or spin column systems and that are suitable to be used in PCR, RT-PCR and qPCR for virus and viroid detection. Because of its easy implementation and the use of safe and inexpensive reagents, it can be easily implemented to work in plant virus and viroid detection in different plant species.
© 2016 Elsevier B.V. All rights reserved |
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Palabras claves : |
DNA; PCR; PLANT VIROID; PLANT VIRUS; PURIFICATION; RNA; RT-PCR. |
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Thesagro : |
CITRUS. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02137naa a2200301 a 4500
001 1055729
005 2019-10-09
008 2016 bl uuuu u00u1 u #d
024 7 $a10.1016/j.jviromet.2016.08.011$2DOI
100 1 $aARRUABARRENA, A.
245 $aApplication of a simple and affordable protocol for isolating plant total nucleic acids for RNA and DNA virus detection.$h[electronic resource]
260 $c2016
500 $aArticle history: Received 30 May 2016 / Received in revised form 26 July 2016 / Accepted 14 August 2016 / Available online 16 August 2016.
520 $aABSTRACT. Standard molecular methods for plant virus diagnosis require the purification of RNA or DNA extracts from a large number of samples, with sufficient concentration and quality for their use in PCR, RT-PCR, or qPCR analysis. Most methods are laborious and use either hazardous and/or costly chemicals.Apreviously published protocol for RNA isolation from several plant species yields high amounts of good quality RNADNA mixture in a simple, safe and inexpensive manner. In the present work, this method was tested to obtain RNA-DNA extracts from leaves of tomato, potato and three species of citrus, and was compared with two commercial kits. The results demonstrated that this protocol offers at least comparable nucleic acid quality, quantity and purity to those provided by commercial phenol-based or spin column systems and that are suitable to be used in PCR, RT-PCR and qPCR for virus and viroid detection. Because of its easy implementation and the use of safe and inexpensive reagents, it can be easily implemented to work in plant virus and viroid detection in different plant species. © 2016 Elsevier B.V. All rights reserved
650 $aCITRUS
653 $aDNA
653 $aPCR
653 $aPLANT VIROID
653 $aPLANT VIRUS
653 $aPURIFICATION
653 $aRNA
653 $aRT-PCR
700 1 $aBENITEZ-GALEANO, M.J.
700 1 $aGIAMBIASI, M.
700 1 $aBERTALMIO, A.
700 1 $aCOLINA, R.
700 1 $aHERNÁNDEZ-RODRÍGUEZ, L.
773 $tJournal of Virological Methods, 2016$gv.237, p. 14-17.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
22/05/2023 |
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Actualizado : |
24/05/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Nacionales |
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Circulación / Nivel : |
Nacional - -- |
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Autor : |
IRISARRI, P.; PEREYRA, V.; FERNÁNDEZ, A.; TERRA, J.A.; TARLERA, S. |
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Afiliación : |
PILAR IRISARRI, Universidad de la República, Facultad de Agronomía, Departamento de Biología Vegetal, Montevideo, Uruguay; VIRGINIA PEREYRA, Universidad de la República, Facultad de Química, Laboratorio de Ecología Microbiana y Ambiental, Montevideo, Uruguay; ANA FERNÁNDEZ, Universidad de la República, Facultad de Química, Laboratorio de Ecología Microbiana y Ambiental, Montevideo, Uruguay; JOSÉ ALFREDO TERRA FERNÁNDEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVANA TARLERA, Universidad de la República, Facultad de Química, Laboratorio de Ecología Microbiana y Ambiental, Montevideo, Uruguay. |
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Título : |
CH4 and N2O Emissions in a Rice Field: First Measurements in the Uruguayan Productive System. [Emisiones de CH4 y N2O en un arrozal: primeras medidas en el sistema productivo uruguayo]. [Special Issue 25 Years Agrociencia]. |
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Complemento del título : |
Plant Biology. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Agrociencia Uruguay, 2022, vol. 26, NE2, e1083. doi: https://doi.org/10.31285/AGRO.26.1083 -- OPEN ACCESS. |
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ISSN : |
2730-5066 |
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DOI : |
10.31285/AGRO.26.1083 |
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Idioma : |
Inglés |
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Notas : |
Article history: Article originally published in: Agrociencia (Uruguay). 2012;16(2):1-10. doi: https://doi.org/10.31285/AGRO.17.533 -- Correspondence: Silvana Tarlera,
starlera@fq.edu.uy -- Special Issue 25 Years Agrociencia. -- License: This work is licensed under a Creative Commons Attribution 4.0 International License. (https://creativecommons.org/licenses/by/4.0/ ) |
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Contenido : |
ABSTRACT.- Irrigated rice fields are major sources of two important greenhouse gases (GHG), methane and nitrous oxide. As an initial step towards obtaining local information, emissions of CH4 and N2O from rice paddy soil were measured by the static chamber technique in greenhouse and field experiments conducted in eastern Uruguay. In the greenhouse experiment, the effect of two flooding moments (21 and 45 days after emergence) and nitrogen fertilization (0 and 50 kg N ha-1) on gas emissions was studied. Early flooding and nitrogen fertilization tended to increase N2O emissions. In the field experiment, effect of winter soil cover crop and nitrogen fertilization (0 and 82 kg N ha-1) were tested. Higher CH4 fluxes were observed mainly during the reproductive stage of the plant in the N-fertilized treatment with ryegrass winter crop. N2O flux peaked at flushing. Results indicate that the use of cover crops might increase GHG emissions during the rice cycle. Despite differences in agronomic management practices employed in Uruguay, CH4 and N2O fluxes are within magnitudes previ-ously reported for rice fields worldwide. .-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-. RESUMEN.- Los arrozales son fuente de dos importantes gases de efecto invernadero (GEI), metano y óxido nitroso. Como un paso inicial hacia la obtención de información local, se midieron las emisiones de CH4 y N2O del suelo y de las plantas de arroz mediante la técnica de la cámara estática en experimentos en invernáculo y a campo en el este de Uruguay. En el experimento en invernáculo, se estudió el efecto del momento de inundación (21 y 45 días después de la emergencia) y de la fertilización nitrogenada (0 y 50 kg N ha-1) sobre las emisiones. La inundación temprana y la fertilización nitrogenada tendieron a aumentar las emisiones de N2O. En el experimento a campo, se estudió el efecto de la cobertura invernal y de la fertilización nitrogenada (0 y 82 kg N ha-1). Se detectaron mayores flujos de CH4 durante la etapa reproductiva de la planta en el tratamiento fertilizado con cobertura invernal previa de raigrás. El flujo de N2O fue máximo después de los baños. Los resultados indican que el uso del cultivo de cobertura podría incrementar las emisiones de GEI durante el ciclo del arroz. A pesar de las distintas prácticas de manejo del cultivo empleadas en Uruguay, los flujos de CH4 y N2O se encuentran dentro de los valores informados previamente para arrozales de otras partes del mundo. Copyright (c) 2022 Agrociencia Uruguay MenosABSTRACT.- Irrigated rice fields are major sources of two important greenhouse gases (GHG), methane and nitrous oxide. As an initial step towards obtaining local information, emissions of CH4 and N2O from rice paddy soil were measured by the static chamber technique in greenhouse and field experiments conducted in eastern Uruguay. In the greenhouse experiment, the effect of two flooding moments (21 and 45 days after emergence) and nitrogen fertilization (0 and 50 kg N ha-1) on gas emissions was studied. Early flooding and nitrogen fertilization tended to increase N2O emissions. In the field experiment, effect of winter soil cover crop and nitrogen fertilization (0 and 82 kg N ha-1) were tested. Higher CH4 fluxes were observed mainly during the reproductive stage of the plant in the N-fertilized treatment with ryegrass winter crop. N2O flux peaked at flushing. Results indicate that the use of cover crops might increase GHG emissions during the rice cycle. Despite differences in agronomic management practices employed in Uruguay, CH4 and N2O fluxes are within magnitudes previ-ously reported for rice fields worldwide. .-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-. RESUMEN.- Los arrozales son fuente de dos importantes gases de efecto invernadero (GEI), metano y óxido nitroso. Como un paso inicial hacia la obtención de información local, se midieron las emisiones de CH4 y N2O del suelo y de las plantas de arroz mediante la técnica de la cámara estática en experimentos en invernáculo y a cam... Presentar Todo |
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Palabras claves : |
Fertilización N; Gases de efecto invernadero; Greenhouse gases; N fertilization; Rice paddy soil; Suelo inundado cultivado con arroz. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/17167/1/2730-5066-1083.pdf
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Marc : |
LEADER 03911naa a2200277 a 4500
001 1064155
005 2023-05-24
008 2022 bl uuuu u00u1 u #d
022 $a2730-5066
024 7 $a10.31285/AGRO.26.1083$2DOI
100 1 $aIRISARRI, P.
245 $aCH4 and N2O Emissions in a Rice Field$bFirst Measurements in the Uruguayan Productive System. [Emisiones de CH4 y N2O en un arrozal: primeras medidas en el sistema productivo uruguayo]. [Special Issue 25 Years Agrociencia].$h[electronic resource]
260 $c2022
500 $aArticle history: Article originally published in: Agrociencia (Uruguay). 2012;16(2):1-10. doi: https://doi.org/10.31285/AGRO.17.533 -- Correspondence: Silvana Tarlera, starlera@fq.edu.uy -- Special Issue 25 Years Agrociencia. -- License: This work is licensed under a Creative Commons Attribution 4.0 International License. (https://creativecommons.org/licenses/by/4.0/ )
520 $aABSTRACT.- Irrigated rice fields are major sources of two important greenhouse gases (GHG), methane and nitrous oxide. As an initial step towards obtaining local information, emissions of CH4 and N2O from rice paddy soil were measured by the static chamber technique in greenhouse and field experiments conducted in eastern Uruguay. In the greenhouse experiment, the effect of two flooding moments (21 and 45 days after emergence) and nitrogen fertilization (0 and 50 kg N ha-1) on gas emissions was studied. Early flooding and nitrogen fertilization tended to increase N2O emissions. In the field experiment, effect of winter soil cover crop and nitrogen fertilization (0 and 82 kg N ha-1) were tested. Higher CH4 fluxes were observed mainly during the reproductive stage of the plant in the N-fertilized treatment with ryegrass winter crop. N2O flux peaked at flushing. Results indicate that the use of cover crops might increase GHG emissions during the rice cycle. Despite differences in agronomic management practices employed in Uruguay, CH4 and N2O fluxes are within magnitudes previ-ously reported for rice fields worldwide. .-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-. RESUMEN.- Los arrozales son fuente de dos importantes gases de efecto invernadero (GEI), metano y óxido nitroso. Como un paso inicial hacia la obtención de información local, se midieron las emisiones de CH4 y N2O del suelo y de las plantas de arroz mediante la técnica de la cámara estática en experimentos en invernáculo y a campo en el este de Uruguay. En el experimento en invernáculo, se estudió el efecto del momento de inundación (21 y 45 días después de la emergencia) y de la fertilización nitrogenada (0 y 50 kg N ha-1) sobre las emisiones. La inundación temprana y la fertilización nitrogenada tendieron a aumentar las emisiones de N2O. En el experimento a campo, se estudió el efecto de la cobertura invernal y de la fertilización nitrogenada (0 y 82 kg N ha-1). Se detectaron mayores flujos de CH4 durante la etapa reproductiva de la planta en el tratamiento fertilizado con cobertura invernal previa de raigrás. El flujo de N2O fue máximo después de los baños. Los resultados indican que el uso del cultivo de cobertura podría incrementar las emisiones de GEI durante el ciclo del arroz. A pesar de las distintas prácticas de manejo del cultivo empleadas en Uruguay, los flujos de CH4 y N2O se encuentran dentro de los valores informados previamente para arrozales de otras partes del mundo. Copyright (c) 2022 Agrociencia Uruguay
653 $aFertilización N
653 $aGases de efecto invernadero
653 $aGreenhouse gases
653 $aN fertilization
653 $aRice paddy soil
653 $aSuelo inundado cultivado con arroz
700 1 $aPEREYRA, V.
700 1 $aFERNÁNDEZ, A.
700 1 $aTERRA, J.A.
700 1 $aTARLERA, S.
773 $tAgrociencia Uruguay, 2022, vol. 26, NE2, e1083. doi: https://doi.org/10.31285/AGRO.26.1083 -- OPEN ACCESS.
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